Ctcf antibody chip

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August undefined, 2024

WebProduct Information. 10915-1-AP targets CTCF in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. … WebPolyclonal Antibody for studying CTCF. Cited in 67 publications. Validated for WB, IP, IF, ChIP. Highly specific and rigorously validated in-house, CTCF Antibody (CST #2899) is …

Antibodies validated for CUT&Tag Assays - Active Motif

WebJan 14, 2024 · As a complement to our genome-wide comparisons of CTCF and cohesin binding, we performed a thorough analysis of CTCF antibody performance in ChIP-seq, finding that a number of antibodies fail to detect the full spectrum of CTCF sites (SI Appendix, Fig. S3). This observation suggests that the high prevalence of CNC sites in … WebChromatin Immunoprecipitation: An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells. Use Anti-CTCF … tsa pre check appointment raleigh nc

CTCF Rabbit pAb-Polyclonal Antibodies - ABclonal

WebThe ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG), and qPCR primers which amplify a 165 bp region of human H19 internal … WebChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9. AbFlex® CTCF antibody (rAb) tested by Western blot. 20 µg of HeLa … tsa precheck at bwi

ChIPAb+ CTCF - ChIP Validated Antibody and Primer Set

WebAntibodies that detect CTCF can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and … WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408) Research with confidence – consistent and reproducible results with every batch. Long … tsa precheck asheville ncWebFeb 12, 2024 · ChIP was performed with 2 µl of rabbit polyclonal antibody crude sera against CTCF 1–293 or Cp190 1–1096, each incubated with half of the chromatin prepared from a biological replicate ... tsa precheck at dia

"WebChIP results obtained with the Diagenode antibody directed against CTCF. ChIP was performed with the Diagenode antibody against CTCF (cat. No. C15410210) on sheared … BACKGROUND: Chromatin immunoprecipitation coupled to … " - Ctcf antibody chip

Ctcf antibody chip

WebChIP-sequencing - Anti-CTCF antibody [EPR7314(B)] - ChIP Grade – BSA and Azide free (ab240035) Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 30 µg of chromatin and 4 µg of ab128873. ChIP DNA was sequenced on the Illumina NextSeq 500 to a depth of 30 … WebAnti-BPTF (VCU19) Antibody, Cat. No. ABE1966, is a highly specific rabbit polyclonal antibody that targets BPTF and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq and Western Blotting applications. Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected BPTF chromatin occupancy at known BPTF …

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WebThis CTCF antibody was raised against a peptide within the N-terminal region of human CTCF. Validations. Orthogonal; Applications. CUT&Tag sequencing (CUT&Tag) Chromatin immunoprecipitation (ChIP) Electrophoretic mobility shift assay (EMSA) Immunocytochemistry (ICC) Immunofluorescence (IF) Immunohistochemistry (IHC) … WebApplications. Use Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor. An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells.

WebCTCF antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa … WebJan 14, 2024 · ChIC/CUT&RUN sequencing for anti-CTCF antibody [EPR18253] - ChIP Grade (ab188408). Figure 2. ChIC/CUT&RUN sequencing for anti-H3K4me3 antibody [EPR20551-225] - ChIP Grade (ab213224). Browse all our ChIC/CUT&RUN-seq validated recombinant antibodies. To learn more about our ChIC/CUT&RUN-seq validated …

WebMay 28, 2024 · Abcam CTCF antibody (Abcam, ab70303) was used in western blot on mouse samples at 1:1000 (fig s5a). Cell Rep (2024) ncbi: domestic rabbit polyclonal. ... Abcam CTCF antibody (Abcam, ab70303) was used in ChIP-Seq on mouse samples (fig 1). Nucleic Acids Res (2024) ncbi: mouse monoclonal (mAbcam 37477) Web( B ) ChIP analysis of CTCF binding at the VEGF proximal promoter. MCF7 cells were cross-linked and sonicated. Sonicated chromatin fragments were precipitated with an anti-CTCF antibody or control ...

WebJan 7, 2024 · b CTCF ChIP-seq data for replicate LNCaP RNAi experiments and wildtype GM12878, K562, ... Antibody/protein complexes were bound to Salmon Sperm …

WebCTCF Rabbit pAb (A12562) Western blot analysis of extracts of various cell lines, using CTCF antibody (A12562) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit … tsa precheck at dfwWebAug 11, 2024 · Using published CTCF ChIP-seq datasets ... for 30 min at 4 °C and then incubated with 40 μL Dynabeads Protein A bound to 10μg of anti-RPA32 antibody (Abcam ab10359) or 10μg of Guinea Pig anti ... tsa precheck at dfw airportWebInvitrogen Anti-CTCF Polyclonal, Catalog # PA5-17143. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF), Immunoprecipitation (IP), ChIP assay (ChIP) and RNA Immunoprecipitation (RIP) applications. This antibody reacts with Human, Mouse, Non-human primate, Rat samples. Supplied as 100 µL purified antibody (14 µg/mL). tsa precheck around meWebThe ChIPAb+ CTCF set includes the CTCF antibody, a negative control antibody (normal mouse IgG), and qPCR primers which amplify a 165 bp region of human H19 internal control region. The CTCF and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of CTCF ... philly cheese steak alma schoolWebUse Anti-CTCF Antibody (Rabbit Polyclonal Antibody) validated in ChIP, WB, ChIP-seq to detect CTCF also known as 11-zinc finger protein, CCCTC-binding factor. An … philly cheese steak american deliWebMar 25, 2011 · To confirm that the observed effects of HDAC inhibition were due to increased histone hyperacetylation at the promoter of Glut-4, we carried out chromatin immunoprecipitation (ChIP) analysis at the Glut-4 promoter in A549 cells treated with TSA. A no-antibody control was included to test for non-specific carriage of DNA with histones. tsa precheck at iahWebCTCF Rabbit pAb (A12562) Western blot analysis of extracts of various cell lines, using CTCF antibody (A12562) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. tsa precheck at atlanta airport