Irf3 phosphorylation antibody

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August undefined, 2024

WebAnti-IRF3 antibody [IRF35I218] (ab50772) Datasheet Reviews ( 3) Q&A (4) References (9) Key features and details Mouse monoclonal [IRF35I218] to IRF3 Suitable for: ICC/IF, WB, Flow Cyt Reacts with: Human Isotype: IgG1 You may also be interested in Primary Alexa Fluor® 488 Anti-PSMA antibody [GCP05] (ab187570) WebPrimary Anti-IRF3 (phospho S386) antibody [EPR2346] - BSA and Azide free (ab182859) Protein Recombinant Human IRF3 protein (ab205216) View more associated products Overview Product name Anti-IRF3 (phospho S386) antibody [EPR2346] See all IRF3 primary antibodies Description Rabbit monoclonal [EPR2346] to IRF3 (phospho S386) Host …

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WebInvitrogen Anti-Phospho-IRF3 (Ser396) Polyclonal, Catalog # 720012. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. This antibody reacts with Human … WebAntibodies that detect IRF3 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry and Peptide array. These antibodies target IRF3 in Human, Mouse, Rat, Non-human primate and Porcine samples. Our IRF3 monoclonal, polyclonal, recombinant monoclonal and recombinant ... north dayton fish market

Phospho-IRF3 (Ser396) Monoclonal Antibody (E.875.8)

WebDec 15, 2014 · To directly observe the distribution of HSPD1 and activated IRF3, an antibody specific to phosphorylated IRF3 (phospho S386) was used. Not surprisingly, overexpression of MAVS for 16 h resulted in phosphorylation of IRF3. Furthermore, almost all of the activated IRF3 co-localized with HSPD1 at that time point . WebKey features and details Rabbit polyclonal to IRF3 Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Western blot … WebMAVS then activates the kinase TANK-binding kinase 1 (TBK1), which phosphorylates IRF3 and IRF7, causing these transcription factors to homodimerize and enter the nucleus to turn on type I IFNs. MAVS also activates the kinase IKKβ, which activates nuclear factor kappa B (NF-κB) to induce proinflammatory cytokines. north dayton discount tire

Recombinant Anti-IRF3 antibody [EPR2418Y] (ab68481) - Abcam

Anti-IRF3 Antibodies Invitrogen - Thermo Fisher Scientific

WebNov 29, 2024 · This phosphorylation event causes IRF3 to dimerize, translocate into the nucleus, and induce the expression of IFN-β. IFN-β then induces the expression of IFN-stimulated genes (ISGs) through... Web), the data reported here suggest that additional signals stimulate phosphorylation of IRF3 and IRF7, essential events in induction of the protective IFN response. This evidence is … north dayton garden centersWebMar 5, 2024 · Rnf39 deficiency and knockdown both enhanced RNA virus–induced IRF3 phosphorylation (Fig. 2, A to C, and fig. S3D). Furthermore, ... The cell lysates were immunoprecipitated with Flag antibody and then immunoblotted with the indicated antibodies. The specific bands were marked by asterisk. All experiments were repeated at … north deal community company ltd

"WebOct 6, 2024 · IRF3 phosphorylation by TBK1 and IKKε leads to its dimerization and nuclear translocation, where it associates with CBP/p300 to initiate transcription of anti-viral target genes, such as type I interferons (TI-IFNs). TRAF3 plays a central role in Toll-like receptor (TLR) signaling through generation of activating polyubiquitin chains. ... " - Irf3 phosphorylation antibody

Irf3 phosphorylation antibody

Phospho-IRF-3 (Ser396) Antibody Cell Signaling …

WebJan 19, 2024 · The phosphorylation of IRF3 plays critical roles in determining the immune state and the cell fate against viral infection. Until now, the sequential activation mode of IRF3 through multi-site phosphorylation remains controversial. ... Antibodies; Rabbit polyclonal anti-IRF3: Santa Cruz Biotechnology: Cat# sc-9082; RRID: AB_2264929: Anti … WebPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members at physiological conditions. Species Reactivity: Human. Species predicted to react based on … Highly specific and rigorously validated in-house, Phospho-IRF-3 (Ser396) (4D4G) …

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WebKey features and details Rabbit polyclonal to IRF3 (phospho S396) Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Primary Anti-IRF3 (phospho S386) … WebApr 14, 2003 · Viral infection results in the phosphorylation of IRF3, leading to its dimerization and interaction with the coactivators CREB-binding protein (CBP) and p300 …

WebTBK1-dependent phosphorylation of IRF3 leads to its nuclear translocation and subsequent activation of transcriptional programs that ultimately lead to ... for 2 h at RT or over-night at 4°C. Primary antibodies used include: anti-pDNA-PKcs Ser2056 (ab124918, Abcam), anti-pDNA-PKcs Ser2056 (ab18192, Abcam) for mouse cell lines, anti-DNA-PKcs ... WebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI …

WebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation. WebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho …

WebMar 4, 2024 · IRF3 is a transcriptional factor that mediates expression of a large array of genes after viral infection. We hypothesized that DUBs might regulate the activity or stability of IRF3 and thereby facilitate or inhibit cellular antiviral responses.

WebMar 23, 2001 · We have investigated the mechanisms of phosphorylation of IRF7 and its close relative, IRF3, during activation of IFN gene expression following Newcastle disease virus (NDV) infection. By all parameters tested, IRF3 and IRF7 appeared to be activated by the same mechanism. north dbh fanartWebOct 22, 2024 · Phosphorylation occurs when IRF3 is recruited to adaptor proteins via a positively charged surface within the regulatory domain of IRF3. This positively charged surface also plays a crucial role in forming the active homodimer by interacting with the phosphorylated sites stabilizing the homodimer. how to respond to interview assignment emailWeb(Microbial infection) Phosphorylation and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3. Ubiquitinated; ubiquitination involves RBCK1 leading to … north dayton ohio hotelsWebPhospho-IRF3 (Ser396) Antibody (MA5-14947) in WB Frontiers in microbiology 2024 - FIGURE 2 Effects of Set-Met on the expression of MAVS protein and the phosphorylation of IRF-3 induced by PDCoV. LLC-PK cells were incubated with PDCoV for 1 h, and 2, 4, 8, and 16 muM of Se-Met was added. north dayton rheumatologyWebNational Center for Biotechnology Information north dearborn library hoursWeb该IRF3 Polyclonal Antibody. This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha … north dearborn crossingWebMar 21, 2024 · IRF3 (Interferon Regulatory Factor 3) is a Protein Coding gene. Diseases associated with IRF3 include Encephalopathy, Acute, Infection-Induced 7 and Encephalopathy, Acute, Infection-Induced 10 . Among its related pathways are Cytosolic sensors of pathogen-associated DNA and MyD88 dependent cascade initiated on … north dayton school of discovery jobs