WebmitoTimer体系：【将Timer基因与线粒体靶向序列结合，获得的MitoTimer蛋白能够在线粒体富集，实现对线粒体状态的检测。 Timer蛋白是红色荧光蛋白dsRed的突变体，其荧光 … WebMitoTimer as a tool for monitoring mitochondrial turnover in vitro and in vivo. 3. Results and discussion 3.1. Constitutive expression of MitoTimer in vitro High membrane potential is required for mitochondrial protein import; thus hypo-polarized mitochondria cannot import newly syn-thesized (green) MitoTimer protein and consequently exhibit a high
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WebJan 1, 2024 · When CAG-CAT-MitoTimer mice were crossbred adiponectin-rtTA-TRE-Cre mice, the double transgenic offspring showed MitoTimer expression revealing mitochondrial network in white adipose tissue following 2 weeks of feeding with doxycline-chow (Fig. 2 e). Similarly, when crossbred with PepCK-Cre mice, the double transgenic offspring showed … WebMitochondrial dysfunction plays important roles in many diseases, but there is no satisfactory method to assess mitochondrial health in vivo.Here, we engineered a MitoTimer reporter …
WebJul 18, 2024 · Roberta Gottlieb’s lab created another mitochondrion targeted fluorescent protein several years ago, called MitoTimer. First developed in 2000, Timer is a fluorescent protein with two point mutations in DsRed that, in the presence of oxygen, changes from green to red. “We modified Timer to incorporate a mitochondrial targeting sequence from ... WebMitoTimer expression was detected as early as 4 h and persisted (in the presence of continuous Dox) for at least 72 h. To overcome the asynchronous incorporation of …
WebMitoTimer reporter gene. Mitochondria are the power plants in mammalian cells for ATP production essential for cellular processes. Mitochondrial dysfunction is associated with most of the prevalent health disorders, including cardiovascular disease, diabetes, cancer, and neurodegeneration. Impaired mitochondrial function often triggers a ... Web(A) heK 293 cells were transfected with pTRe-MitoTimer (MT), exposed to Dox (+) or vehicle (−) for 24 h, then subjected to subcellular fractionation to obtain mitochondria (lanes 1-3) and ...
WebJan 1, 2014 · The MitoTimer fluorescent protein has been described in recent studies as a new probe to measure different aspects of mitochondrial turnover and efficacy of quality control ( Ferree et al., 2013, Hernandez et al., 2013, Laker et al., 2014). MitoTimer consists of the Timer fluorescent protein fused to the mitochondrial targeting sequence of ...
WebMitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS ... pytorch load bert modelWebMitoTimer: A novel tool for monitoring mitochondrial turnover. Hernandez G, Thornton C, Stotland A, Lui D, Sin J, Ramil J, Magee N, Andres A, Quarato G, Carreira RS ... pytorch load evalWebMay 1, 2016 · The MitoTimer transgene combines the red fluorescent protein, dsRed, and a human cytochrome c oxidase subunit VIII (Cox 8) mitochondrial localization sequence to traffic the protein to the mitochondria where the color shifts from green to red over time (approximately 24 hours). Our goal is to use this as a tool to assess the increase or ... pytorch load h5WebConclusion: MitoTimer is useful for assessment of mitochondrial health in vivo. Significance: MitoTimer could advance mitochondrial research in multiple disciplines. Mitochondrial … pytorch load map_locationWebOct 11, 2013 · Flow cytometry analysis of MitoTimer in cells. ( A) Transfected cells were exposed to Dox for 1 h, then cultured for the indicated times (4–144 h), harvested, and … pytorch load hdf5Web(A) heK 293 cells were transfected with pTRe-MitoTimer (MT), exposed to Dox (+) or vehicle (−) for 24 h, then subjected to subcellular fractionation to obtain mitochondria … pytorch load map locationWebJan 1, 2016 · MitoTimer reveals mitochondrial turnover at the level of individual mitochondria: fluorescence-activated organelle sorting (FAOS) of mitochondria from … pytorch load huge dataset