Phis2 酵母单杂

Webb12 apr. 2024 · 方法：以启动子a构建到酵母单杂交启动子载体phis2，文库质粒为pgadt7-中山杉文库。 材料： 1．启动子克隆：pHIS2-A。 根据实验需求进行扩增并酶切构建载 … Webb产品名称：pHIS2酵母单杂交质粒. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was …

pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

http://www.zoonbio.com/molecular/yeast-one-hybrid-technology.html Webb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍；并以Y1Hgold（pAbAi-bait）和Y187（pHis2-bait）为例，介绍了自激活检测的目的以及主要 … sims with no blur https://rockandreadrecovery.com

pHIS2酵母单杂交质粒_价格-品牌-详情介绍_丁香通

http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html http://muchong.com/t-9775583-1 http://www.zoonbio.com/molecular/yeast-one-hybrid.html rct food

pHIS2酵母单杂交质粒_价格-品牌-详情介绍_丁香通

Category:Y187-pHis2 Yeast One-Hybrid Media Kit Y187-pHis2 酵母单杂培养 …

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Phis2 酵母单杂

pHIS2说明书-分析方法-生物在线 Lab-on-Web - BIOON

http://www.honorgene.com/product_list/carrier_library/123112.html WebbPhis2osgpx1 Promoter Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2 1. phis2 1 vector TaKaRa is a verified supplier TaKaRa ...

Phis2 酵母单杂

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http://www.bioon.com.cn/reagent/show_product.asp?id=3900880 Webb1 mars 2024 · VvACTIN or AtACTIN2 was used as internal references for grape and Arabidopsis. The qRT-PCR primers are listed in Table 1. Table 1. Gene-specific primers used for quantitative real-time PCR assays. 2.7. Transformation of Arabidopsis The 35S: VvWRKY30 vector was created by cloning genes into the pSuper1300 binary vector with …

Webb1 apr. 2024 · The Y187 strain was prepared for the Y1H assay, we mixed pAD-CmBBX7 and pAD-GUS with pHIS2-CmFTL1pro respectively, and transformed them into Y187 strain using the lithium acetate method.Then inoculated on SD/His-Trp-Leu-plates, and screened for the lowest concentration range of 3-AT that was sufficient to inhibit the growth of … http://zomanbio.com/shengwu_info.php?nid=2549

Webb酵母单杂交技术就是依据这个原理，通过构建可与转录激活结构域结合的蛋白，然后与特异的DNA序列相结合，进而启动下游报告基因的表达。 目前酵母单杂交体系已被广泛应用于验证DNA与蛋白质的相互作用，寻找与目的DNA片段相互作用的蛋白质分子，由此找到了许多新的转录因子。 酵母单杂交技术的优势 ①酵母单杂交技术简单易行，在这个过程中，可 … Webb9 juli 2024 · 幼儿/小学教育 -- 教育管理. 文档标签：. phis2酵母表达载体说明. 系统标签：. 酵母表达载体 多克隆位点 载体 克隆方法 说明 拷贝. pHis2编号载体名称北京华越洋生物VECT2110pHis2pHis2载体基本信息出品公司:Clontech载体名称:pHis2质粒类型:酵母单杂交载体高拷贝/低拷贝 ...

Webb1 dec. 2024 · AD/pATM1-pHIS2 and AD-ATM1/pATM1-pHIS2 were cotransformed into the Y187 strain and cultured on SD/-Trp/-Leu for 2–3 days at 30 °C. Then, healthy and consistent clones were diluted, ...

Webb1、pHis2-Bait或pAbAi-Bait和AD-prey质粒 2、或者Bait和Prey序列，质粒由我们构建 1、提供详细的、完整的实验报告，清晰的实验图片 2、在受体菌的阳性菌 自激活和毒性验证: … rct for diabetesWebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: yeast series, yeast one hybrid vector Plasmid size: 7207bp … sims wixsiteWebb还有一点，我看了pHIS2的序列，在HIS3前面，Pmin区域里也有我的转录因子结合位点，会不会是我的转录因子结合到那里去了？不知道有没有这个可能性啊？这个minimum … sims with modsWebbpHis2酵母单杂交载体是由Biovector Co.,LTD代理或销售的Biovector, Addgene, ATCC, Invi品牌的试剂，产品来源于Biovector Inc. USA。Biovector Co.,LTD是中国最权威的pHis2酵母单杂交载体试剂销售服务商之一，在海淀区等地方销售pHis2酵母单杂交载体试剂已经多年。生物在线为您提供众多企业pHis2酵母单杂交载体仪器产品及 ... sims witchcrafthttp://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html sims with ccWebb2 aug. 2024 · 酵母单杂交技术是在酵母双杂基础上发展而来的一种研究核酸-蛋白相互作用的工具，被广泛用于研究真核细胞内基因的表达调控，如鉴别DNA结合位点发现潜在的 … rct food waste collectionWebb22 juli 2013 · 酵母单杂交系统 Clontech pHis2说明书 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit （#K1617-1）. sims witchy cc